Description
PH1859 SSC buffer (20×, pH7.0) SSC Buffer 100mL/500mL
PH1860 SSC buffer (20×, pH7.4) SSC Buffer 100mL/500mL
PH1861 SSC buffer (20×, pH5.3) SSC Buffer 100mL/ 500mL
SSC (saline sodium citrate) buffer solution is the most standard blotting and hybridization treatment solution in molecular biology. It is designed to be used in various hybridization experiments to achieve denaturation and cleaning purposes. The main components are sodium chloride and sodium citrate. The sodium citrate in the SSC buffer acts as a buffer. The salt ion (Na) neutralizes the negative charge on the nucleic acid backbone and makes it neutral. This makes the probe and target sequence easier to bind. It is also used in the preparation of SDS-PAGE electrophoresis separation gel. For nucleic acid hybridization, different concentrations have different effects: commonly used 2×, and 0.5×
2×SSC: high-salt washing membrane, washing away some non-specific binding probes.
0.5×SSC: Low-salt washing membrane increases the stringency of the nucleic acid chain and increases the repulsive force between RNA/DNA.
This product is a 20× concentrated solution, which can be directly diluted with deionized water according to experimental requirements.
Transportation and storage: Store at room temperature, valid for 12 months.
Note:
1. Pay attention to avoid contamination of the solution by microorganisms during use.
2. Store at room temperature. If the solution is stored at low temperature, precipitation may occur, which can be dissolved by shaking or heating.
In biochemistry and molecular biology, the saline-sodium citrate buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.