Tris boric acid buffer
Tris boric acid electrophoresis buffer (10×TBE) TBE Buffer, 10×
Specification 10X TBE (Customizable), pH (Customizable)
Pack size: 100 mL | 200 mL | 500 mL |1000mL (Customizable)
Store at RT, valid for 12 months.
TBE buffer Product Introduction
TBE buffer is a nucleic acid electrophoresis buffer salt solution commonly used in biology, mainly used for agarose gel electrophoresis of DNA. The main components of TBE are Tris-borate and EDTA, with strong buffering capacity, suitable for longer electrophoresis, high resolution, and good separation effect when electrophoresing fragments less than 1kb. The boric acid component in the TBE buffer will affect the efficiency of DNA recovery and subsequent enzymatic reactions. For agarose gel electrophoresis recovery experiments of DNA fragments, it is recommended to use TAE buffer.
About TBE Buffer
Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (around 1 mM concentration).
Although TBE and TAE are common electrophoresis buffers, there are other options for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE are that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.
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