Tris (Tris(hydroxymethylaminomethane)) is a weak base with a molecular formula of C4H11NO3, a relative molecular weight of 121.14, and a pKa of 8.1 at 25°C. The effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is around 10.5. Generally, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of this pH. It is often used as a biological buffer, and its pH value is 6.8, 7.4, 8.0, 8.8, and its pH value varies greatly with temperature. Generally speaking, every time the temperature increases by 1℃, the pH value decreases by 0.03. 1M Tris-HCl pH8.0 is one of the most commonly used reagents in molecular biology, used as the basic component of various buffers to adjust the pH, such as Prepare Tris-EDTA solution, TBE running buffer, etc.
Tris-HCl buffer (1mol/L, pH8.0, sterile) is prepared with imported Tris and deionized water, and is autoclaved at 15psi. It is widely used as a solvent for nucleic acids and proteins.
Operation steps: operate according to the specific requirements of the experiment.
It is irritating to human body, please pay attention to proper protection.
For your safety and health, please wear lab coats and disposable gloves.
Validity period: stored at room temperature, valid for 12 months.
Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.
“Tris” is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.