Tris EDTA (TE) Buffer
Tris EDTA (TE) Buffer (pH 8.0)
Specification TE (Customizable), pH 8.0 (Customizable)
Pack size: 100 mL | 200 mL | 500 mL | 1000mL (Customizable)
Store at RT, valid for 12 months.
Tris EDTA (TE) Buffer Product Introduction
TE stands for Tris-EDTA buffer, also known as T10E1 buffer. It is a commonly used buffer solution in molecular biology, especially when it involves DNA and RNA to protect it from degradation. TE buffer is composed of two reagents: Tris (the most commonly used pH buffer) and EDTA (divalent metal ion chelating agent). The pH buffer range of the former is 7.5-8.5, and the activity of nuclease is low in this environment; The latter further inactivates the nuclease by chelating metal ions necessary for the nuclease.
This product is a ready-to-use 1×TE buffer, pH 8.0, after autoclaving, does not contain DNase enzyme, and is suitable for DNA purification and storage. It can also be used as a washing buffer.
Product properties 1×TE working solution contains 10 mM Tris-HCl and 1 mM EDTA·Na2, pH 8.0
Tris EDTA (TE) Buffer Precautions
For your safety and health, please wear lab coats and disposable gloves.
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. “TE” is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA while protecting it from degradation.
Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.
Short-term storage (weeks) 4 degree centigrade in Tris-EDTA
Medium-term storage (months) -80 degree centigrade in Tris-EDTA
Long-term storage (years) -80 degree centigrade as a precipitate in ethanol
Long-term storage ( decades) -164 degree centigrade or dried.
Tris EDTA (TE) Buffer 10X Preparation and Recipe
TE Buffer 10X:
Change the value in the textbox above to scale the recipe volume
|Tris-Cl (desired pH) (mw: 157.594 g/mol)||15.759 g||0.1 M|
|EDTA (pH 8) (mw: 292.24 g/mol)||2.92 g||0.01 M|
- Prepare 800 mL of distilled water in a suitable container.
- Add 15.759 g of Tris-Cl (desired pH) to the solution.
- Add 2.92 g of EDTA (pH 8) to the solution.
- Add distilled water until volume is 1 L.